Combine the advantages of rationally designed shRNA with selectable promoters and reporters to perform functional screens of hundreds or thousands of shRNAs in dividing and non-dividing cells.
Innovative vector design for robust shRNA expression from SMARTvector Lentiviral shRNAs
SMARTchoice options give you a choice of promoter and reporter (or no reporter!) for optimal performance in your experiment
Rationally designed shRNAs for maximum knockdown and minimal off-targeting
Choose SMARTvector Inducible Lentiviral shRNA for tight regulation of expression from the Tet-On® 3G transactivator protein
Each SMARTvector Lentiviral shRNA Library contains
≥ 5 x 108 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs
16 x 25 µL (400 µL total) for libraries > 5000 constructs
100 Non-targeting control constructs
272 constructs targeting 34 mouse or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes.
A data file containing construct sequences, target gene IDs, and counts per millions of mapped reads
SMARTvector Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for mouse and mouse; rat libraries and custom SMARTvector Lentiviral shRNA collections are available upon request.
Request a custom collection of mouse mouse, or rat gene-targeting shRNAs
Available in pool sizes between 50 to 10,000 constructs
Pools are provided as purified, concentrated lentiviral particles
≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors
Request pricing for a custom pooled library
Products recommended in our validated protocol:
Vector-matched SMARTvector Non-targeting control for transduction optimization
SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias
High-throughput multiplexed Next Gen Sequencing for hit identification
Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required:
SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual
Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet
SMARTvector Lentiviral shRNA pooled library coverage*:
*Annotations are based on RefSeq v65; coverage and shRNA counts are subject to change without notice
** Clone and gene counts available by request
Collection
mouse
Mouse
# targeted genes
Number pools and average shRNA per pool
# targeted genes
Number pools and average shRNA per pool
Genome 19241 16 pools of 9513 shRNA 21745 18 pools of 9502 shRNA
Druggable Genome 7341 6 pools of 9735 shRNA 9723 8 pools of 9670 shRNA
GPCR 377 1 pool of 3012 shRNA 494 1 pool of 3909 shRNA
Ion Channel 340 1 pool of 2709 shRNA 332 1 pool of 2646 shRNA
Phosphatase 245 1 pool of 1948 shRNA 268 1 pool of 2141 shRNA
Protease 466 1 pool of 3702 shRNA 529 1 pool of 4206 shRNA
Protein Kinase 702 1 pool of 5602 shRNA 697 1 pool of 5558 shRNA
Ubiquitin Conjugation 557 1 pool of 4431 shRNA 511 1 pool of 4069 shRNA
Apoptosis **
Cell Cycle Regulation **
De-ubiquitinating Enzymes **
Membrane Trafficking **
DNA Damage Response **
Epigenetics **
Nuclear Receptor **
Transcription Factors **
Important Notice
The SMARTvector Lentiviral shRNA Pooled Libraries are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the containment measures stated below and in applicable laws and regulations are met. Products may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to mouses for any purpose or to animals for therapeutic purposes. SMARTvector Lentiviral shRNA Pooled Libraries are provided as lentiviral particles are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious mouse disease).
Any investigator who purchases lentiviral particle products is responsible for consulting with their institution's health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.
Caracteristicas
Constitutive shRNA expression for optimized functional analysis screens
