shMIMIC Inducible Lentiviral microRNA Pooled Libra
Disponible
Combine the advantages of selectable promoters on optimized microRNA constructs with the Tet-On 3G tetracycline-inducible expression system for unsurpassed control of mature microRNA expression.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | Librerías miRNA |
Subcategoría de Producto | Mimic |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Ratón |
Especificaciones | Innovative vector design for robust microRNAs robust expression from shMIMIC Inducible Lentiviral microRNAs SMARTchoice options let you select the promoter and reporter for optimal performance in your experiment Tight regulation of expression from the Tet-On® 3G transactivator protein Choose shMIMIC Lentiviral microRNA Pooled Libraries for constitutive expression of mature microRNAs. Each shMIMIC lentiviral library includes: 30 Non-targeting controls 80 species-specific positive control shRNAs targeting 10 common viability and reference genes (8 shRNAs each) A data file including construct sequences, miRBase gene IDs, and counts per millions of mapped reads 1Total constructs includes 110 non-targeting and gene-specific shRNA constructs. All shMIMIC Lentiviral microRNA pooled libraries are ≥ 5 × 108 TU/mL (+/- 20 %) provided in 8 tubes × 25 µL (200 µL total) shMIMIC microRNA Pooled Library Number of mature microRNAs Number of unique designs Number of pools × number of constructs per pool1 Human 2580 2555 1 pool × 2665 constructs Mouse 1913 1896 1 pool × 2006 constructs Human-Mouse Conserved 355 human + 341 mouse 386 1 pool x 496 constructs Custom collections of microRNA pooled libraries are available Request a custom collection of human, mouse, or rat microRNAs Available pool sizes are between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors Request pricing for a custom pooled library Products recommended in our validated protocol: Vector-matched SMARTvector Inducible Non-targeting control for transduction optimization SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias High-throughput multiplexed Next Gen Sequencing for hit identification |
Caracteristicas | Tight control of microRNA expression for powerful functional screening of hundreds or thousands of microRNAs. |
Marca | HORIZON |
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