KIT DIAGNÓSTICO ELISA, PVY, POLICLONAL PARA 960 ENSAYOS, 1 KIT
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Disponible
- In PVY of potatoes, several strain groups are distinguished according to the symptoms on Nicotiana tabacum, Physalis floridana, Solanum tuberosum and other potato cultivars (4). The main groups are: the common (PVYO), tobacco veinal necrosis (PVYN), and stipple streak (PVYC) strain groups of PVY. More recently, subgroups within the historical groups have been described. For example within the PVYO group, atypical isolates, originating mostly from pepper and tobacco, have been assigned to the subgroup PVYOb, whereas the common European potato isolates are called PVYOa (8).
- Within PVYN, subgroups are represented by more recently described isolates such as PVYNTN (involved in the potato tuber necrotic ringspot disease) (9) and the Wilga type PVYNW (2). The latter one (PVYNW) is biologically similar to the «old» N-type (inducing typical necrosis on tobacco), but serologically similar to the O-type (no reaction with our «PVY-necrotic» reagent). Details of detection of these isolates with our PVY reagents are given below in the section specificity and are illustrated in Table 1. BIOREBA AG has collaborated since 1980 with the Swiss Federal Research Station Agroscope Changins-Wädenswil (ACW, Nyon), and other institutes elsewhere for the development of ELISA reagents (7, 8).
- These collaborations allow us to adjust our reagents to new knowledge and new findings as needed. In this regard, we, in cooperation with the ACW, were able to modify and thus optimize our full-spectrum PVY reagent («PVY monoclonal cocktail») introduced many years ago (for details see below). We rely as much as possible on monoclonal antibodies (Mab) for our reagents, since Mab provide several advantages over polyclonal antibodies (Pab): The reagent based on Mab guarantees a well defined and standardized product (constant in specificity and avidity), whereas Pab-based reagents inevitably introduce some variability between batches originating from different animals.
- Once developed, Mab are reproducible year after year. Different complementary Mab can be mixed in a controlled manner in order to broaden its serological range as it has been done for example with our «PVY monoclonal cocktail». This approach using Pab may lead to increased non-specific reactions. On the other hand, Mab are suitable for differentiating PVYN subgroups (example described in section D below). Pathogen-specific Mab do not contain antibodies against host plant proteins that might interfere in the test. Therefore, non-specific background in ELISA is generally decreased, rendering the test more sensitive (wider OD ratio between infected and healthy tissue). But there are exceptional cases where non-specific reactions with Mab do occur (non-specific reactions with certain plant components?). Our reagents have been extensively tested in Europe and at the International Potato Center (CIP) in Peru and were found to be very efficient (W. Bitterlin and P. Gugerli, unpublished; COST 88 PVY ring-test, unpublished; 5, 7, 10). Furthermore, they have been used since the early 1980’s by many scientists and in numerous potato certification laboratories in many parts of the world.
Aplicaciones | Diagnóstico vegetal |
---|---|
Categoría de Producto | Test diagnóstico de plantas |
Subcategoría de Producto | ELISA |
Formato Especial | 96 poc. |
Disponibilidad | Bajo pedido |
Patógeno/Antígeno | PVY-poly |
Especificaciones |
|
Marca | BIOREBA |
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