Accell SMARTpool
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Disponible
A novel siRNA for difficult-to-transfect cells, Accell siRNA is modified to require no transfection reagent or viral vector for delivery. Available as genome-wide, predesigned SMARTpool and single siRNA reagents for human, mouse, and rat.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | Reactivos |
Subcategoría de Producto | siRNA Accell |
Temperatura Envío | Ambiente |
Temperatura Almacenaje | Congelado -20º |
Formato Especial | SMARTpool |
Disponibilidad | Bajo pedido |
Especie/Huesped | Rata |
Especificaciones | Accell siRNA enters cells without the need for transfection reagents, virus (or viral vectors), or instruments Novel siRNA modifications facilitate uptake, stability, specificity and knockdown efficiency Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types Appropriate for use in vivo, due to stabilizing modifications Extended-duration knockdown with optimized continuous application Experimental considerations This breakthrough in siRNA delivery requires no transfection reagent, but has some unique application requirements. Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended Full-serum media can be added back after 48 hours of incubation, optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown Product Formats SMARTpool: A mixture of 4 siRNA provided as a single reagent, providing advantages in both potency and specificity Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene Individual siRNAs: Select 1, 2, 3, or 4 individual siRNAs per gene Sequence information is provided with purchase of predesigned siRNA products |
Caracteristicas | Targeted gene silencing in difficult-to-transfect cells |
Marca | HORIZON |
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